Because four clubs from west of the Great Belt relegated from the 2018–19 Danish 2nd Divisions, and no teams east of the Great Belt, the 8th, 9th and 10th places of the West Groups (3 & 4) faced direct relegation to the lower tiers.
[1] Kastrup Boldklub became to only East Group club to relegate after a 3–0 loss to Taastrup FC in a direct play-off match for a place in the 2019–20 Denmark Series.
TFC Odsherred disbanded following the season, saving Kastrup Boldklub from direct relegation.
Six clubs relegated directly from the Denmark Series from the West Groups, as four clubs west of the Great Belt relegated from the 2018–19 Danish 2nd Divisions (Odder IGF, Tarup-Paarup IF, Kjellerup IF and BK Marienlyst).
The East Region (Groups 1 & 2) relegation play-off was played on 23 June 2019 at the Glostrup Stadion.