The 2019–20 Club Universidad Nacional season is the 65th season in the football club's history and the 58th consecutive season in the top flight of Mexican football.

Players and squad numbers last updated on 3 December 2019.

[1]Note: Flags indicate national team as has been defined under FIFA eligibility rules.

Players may hold more than one non-FIFA nationality.Ordered by squad number.

Source: LigaMX Last updated: 2 December 2019Source: Mediotiempo.com Last updated: 2 December 2019Source: LigaMXOnly competitive matches = Number of bookings; = Number of sending offs after a second yellow card; = Number of sending offs by a direct red card.