However, the demotion of Yeovil Town from the WSL to the third tier FA Women's National League[2] meant that, though the competition would have its highest ever number of participants, one group would still have to contain only five clubs.

The draw for this round was made live via Talksport 2 on 16 December 2019 with the winners of each group all receiving home advantage.

The round took place on Wednesday 15 January 2020 and contained two FA Women's Championship teams, double the amount that reached the quarter-finals the previous season.

The draw for this round took place on 18 January 2019 and was broadcast live on BT Sport Score.

On 16 January, it was announced the 2020 FA Women's League Cup Final would be held at the City Ground, the home of Nottingham Forest, for the first time.