The fixtures were published in June 2019 and the season began on 3 August 2019.

[3] Ten teams contested the league: Airdrieonians, Clyde, Dumbarton, East Fife, Falkirk, Forfar Athletic, Montrose, Peterhead, Raith Rovers and Stranraer.

On 13 March 2020 all SPFL leagues were indefinitely suspended due to the COVID-19 pandemic.

[4] On 8 April, with the pandemic continuing, the SPFL board proposed to curtail the 2019–20 League One season and use the points per game earned by each team to date as the final standings.

Teams play each other four times, twice in the first half of the season (home and away) and twice in the second half of the season (home and away), making a total of 180 games, with each team playing 36.