The 49ers played their home games at Jerry Richardson Stadium in Charlotte, NC, and competed in the East Division of Conference USA (C–USA).

[3] Also coming over on the same date from Austin Peay was Max Thurmond who would take over the same positions of special teams coordinator and linebackers coach.

[3] Carter Crutchfield would also follow Healy from Austin Peay to continue as his recruiting coordinator and director of operations.

[3] On January 8, 2019 Minnesota Defensive line coach Marcus West was reported as taking the same position with the 49ers and also joining Cooper as co-defensive coordinators.

The radio announcers are "Voice of the 49ers" Matt Swierad with play-by-play alongside NFL veteran Al Wallace providing color commentary and Bobby Rosinski and Walker Mehl with sideline reports.

Swierad also hosts the "Niners Live" Coaches Show each Monday during the season at 7:00 pm from Cabarrus Brewing Company.

[37] Conference USA released their preseason media poll on July 16, 2019, with the 49ers predicted to finish in last place in the East Division.

Top performers included Charlotte quarterback Chris Reynolds who passed for 136 yards, a touchdown, and an interception.

After not scoring a touchdown in the previous matchup between these squads the 49ers would manage to keep the game within a two-score margin for the entire high-scoring contest.

Game notes: Charlotte visited "Death Valley" for the first time to meet the defending National Champions.

Top performances were dominated by Clemson and included quarterback Trevor Lawrence who passed for 94 yards and 2 touchdowns.

Game notes: Charlotte and Florida Atlantic both opened conference play in a rematch of the previous season finale.

Game notes: In this first ever meeting of conference foes the Mean Green took an early lead and held it throughout the game; but an offensive flurry for the 49ers in the fourth quarter, combined with the defense shutting down North Texas's high power offense in the same time period, resulted in the Niners gaining their first conference victory of the year.

Game notes: Middle Tennessee would get the first points, but Charlotte's defense wouldn't let them score again until the fourth quarter.

Game notes: The Miners, looking for their first FBS and conference win of the season, would strike quickly by going up two touchdowns in the first quarter and taking a two score lead into the half.

The 49ers would reverse the action in the second half, holding the Miners scoreless while coming from behind to take their first road victory of the year.

Chris Reynolds would have another outstanding performance, setting a new FBS school single game record with 354 passing yards which resulted in 2 touchdowns and an interception.

Behind Quarterback Chris Reynolds the 49ers would stage a second half comeback victory to secure the program's first winning season and first bowl game qualification.

Charlotte would cruise to a relatively easy win over the struggling Monarchs and prepare for their first ever game past the month of November.

Top performers of the game included Charlotte quarterback Chris Reynolds, who threw for 198 yards with a touchdown and an interception.