[2] As was the case at the end of the previous season, there will be limited attendance from the fans in the stadiums due to measures related to COVID-19 pandemic.
[4][5] With Slavia, Viktoria, and Sparta finishing top-three in the 2019–20 season, many expected a close race for the title amongst this trio.
Sparta and Slavia started the season in strong form with unbeaten runs in their first six matches, while Viktoria lost in the second round in Liberec.
The race for the title resumed behind closed doors in November with Viktoria's 3–1 win over Sparta in the seventh round and opened up a lead for the rivals Slavia.
The last match of the autumn program was played on 23 December, the latest such date in Czech league history.
[7] On 8 May 2021, Opava was confirmed to be the first team to be relegated to the FNL after a 1–1 draw at Zlín, with three games remaining ending their three-year tenure in the first league.
[8] A week later on 16 May 2021, 15th-placed Teplice defeated České Budějovice and relegated Brno, immediately returning to the FNL after a season's presence in the top flight.
[9] On 23 May 2021, Příbram was the third and final team to be relegated following a 0–1 defeat at home against Pardubice with one game remaining, also ending their First League tenure after three-years.
FK Příbram were in last position, with elimination playoff spots held by MFK Karviná and SFC Opava.
On 11 July 2020 in the penultimate round of the season FK Pardubice beat Vyšehrad 4–1 and secured automatic promotion to the First League for the first time in their history.