As a result of the COVID-19 pandemic, the start of the 2020–21 season was delayed, forcing many leagues and competitions to shorten their format.
With six group winners now, only the two best second place teams qualified for the knock-out round.
The draw for this round was made live on BT Sport Score on 19 December 2020.
[6] The two teams that qualified as best placed runners-up could not be drawn against the teams that topped their respective groups meaning Bristol City were not eligible to face Crystal Palace and Durham could not be drawn with Aston Villa.
[7] On 28 January, it was announced the 2021 FA Women's League Cup Final would be held at Vicarage Road, the home of Watford FC, for the first time.