During the 2020–21 season, Jong Ajax participated in the Dutch Eerste Divisie, the 2nd tier of professional football in the Netherlands.

It was their 8th consecutive season in the Eerste Divisie.

Note: Flags indicate national team as defined under FIFA eligibility rules; some limited exceptions apply.

For a list of all Dutch football transfers in the summer window (1 July 2020 to 31 August 2020) please see List of Dutch football transfers summer 2020.

For a list of all Dutch football transfers in the winter window (1 January 2021 to 1 February 2021) please see List of Dutch football transfers winter 2020–21.