It is the club's fourth consecutive season in the second division, since relegation from the Bundesliga in 2016–17.

In addition to the domestic league, Darmstadt also participated in this season's edition of the DFB-Pokal.

The season covers the period from 1 July 2020 to 30 June 2021.

Note: Flags indicate national team as defined under FIFA eligibility rules; some limited exceptions apply.

Players may hold more than one non-FIFA nationality.