The winner of the Slovak Cup earned automatic qualification for the second qualifying round in UEFA Europa Conference League.
The Cup was played as a knockout tournament contested by 229 teams - one more than the previous edition.
[5] If the score is still level after ninety minutes, a team advancing to next round will be reached by penalty shoutout.
Only one match including DAC Dunajská Streda was rescheduled on a spring 2021 due COVID-19 pandemic and participation in Europa League.
[8] On March 10, the organizers Slovak Football Association decided that the competition would be played only with clubs from the first and second leagues.
6 teams, selected by worst places from 2019–20 Slovak Cup, played an additional play-off in the 4th round.