The last season champion Viktoriya Mykolaivka are defending their title since declining their opportunity for promotion to professional competitions.
Notes: To the stage qualify eight teams, selection of which is determined exclusively by the AAFU Commission in conducting competitions.
[9] Six teams from AAFU are expected to be promoted to the 2021–22 season in Professional Football League (Article 18 of regulations).
[10] In early December 2020 it became known that at least 10 teams expressed their intention to receive club's license of the Professional Football League for the next season: MFA Mukachevo, Karpaty Lviv, Nyva Terebovlia, Atlet Kyiv, AFSC Kyiv, Viktoria Mykolaivka, Liubomyr Stavyshche, SC Poltava, FC Sumy, FC Trostianets.
[12] Among them are Karpaty Lviv (Tlumak's), MFA Mukachevo, LNZ-Lebedyn, Livyi Bereh Kyiv, Lyubomyr Stavyshche, AFSC Kyiv, FC Sumy, Skoruk Tomakivka, SC Poltava, Viktoriya Mykolaivka, FC Trostianets, Rukh-2 Lviv, Lviv-2, Kryvbas-2 Kryvyi Rih.