The 2020 I.ČLTK Prague Open is a professional tennis tournament played on outdoor clay courts.

It was part of the 2020 ATP Challenger Tour.

It took place in Prague, Czech Republic between 17 August and 6 September 2020.

The following players received wildcards into the singles main draw: The following players received entry into the singles main draw using a protected ranking: The following players received entry from the qualifying draw: