The 2020 Missouri Tigers softball team represented the University of Missouri in the 2020 NCAA Division I softball season.

The Tigers played their home games at Mizzou Softball Stadium.

The Tigers finished the 2019 season 35–25 overall, and 12–12 in the SEC to finish in a tie for sixth in the conference.

The Tigers went 3–2 in the Los Angeles Regional during the 2019 NCAA Division I softball tournament.

The SEC preseason poll was released on January 15, 2020.