In addition to the domestic league, FC Twente participated in this season's edition of the KNVB Cup.

Note: Flags indicate national team as defined under FIFA eligibility rules; some limited exceptions apply.

Players may hold more than one non-FIFA nationality.

Note: Flags indicate national team as defined under FIFA eligibility rules; some limited exceptions apply.

Source: Eredivisie The league fixtures were announced on 11 June 2021.