During the 2021–22 season, Jong Ajax participated in the Dutch Eerste Divisie, the 2nd tier of professional football in the Netherlands.

Note: Flags indicate national team as defined under FIFA eligibility rules; some limited exceptions apply.

Players may hold more than one non-FIFA nationality.

For a list of all Dutch football transfers in the summer window (1 July 2021 to 31 August 2021) please see List of Dutch football transfers summer 2021.

For a list of all Dutch football transfers in the winter window (1 January 2022 to 1 February 2022) please see List of Dutch football transfers winter 2021–22.