In addition to the domestic league, Juventus U23 also played this season's edition of the Coppa Italia Serie C. On 7 August 2021, Juventus U23 played a friendly against Pro Vercelli, winning 4–0.
In the 4th minute Matías Soulé scored the opening goal giving Juventus U23 the lead.
11 minutes later, Belgianmen Moutir Chajia scored the equalizing goal.
[4] Juventus U23 started the competition as defending champions having won the cup in the 2019–20 season winning 3–2 against Pro Sesto.
[6] In the 108th minute, in the extra-time, Fabio Miretti gave the lead to Juventus U23 through a penalty kick.