Leyton Orient announced friendly matches against Dundee,[45] West Ham United,[46] Tottenham Hotspur,[47] Maidenhead United,[45] Dulwich Hamlet,[48] Gillingham,[49] and Heybridge Swifts[45] as part of their pre-season preparations.

Source: [citation needed] The U's fixtures were announced on 24 June 2021.

[54] Orient were drawn at home to Queens Park Rangers in the first round.

[55][56] Leyton were drawn into Southern Group G alongside Charlton Athletic, Crawley Town and Southampton U21s.

The list is sorted by squad number when total goals are equal.