Note: Flags indicate national team as has been defined under FIFA eligibility rules.

Preston North End announced they would play friendlies against Bamber Bridge,[51] St Johnstone,[52] Celtic,[53] Bolton Wanderers,[54] Accrington Stanley,[55] Manchester City,[56] Wigan Athletic[51] and Manchester United[57] as part of their pre-season preparations.

Source: [citation needed] PNE's fixtures were announced on 24 June 2021.

[62] Preston North End were drawn away to Mansfield Town[63][64] and Morecambe[65][66] in the first and second round respectively, then at home against Cheltenham Town[67][68] and Liverpool[69] in the third and fourth round.

Preston North End were drawn away to Rochdale in the first round.