On 11 June 2021, Valeriy Luchkevych was signed on a free transfer from Oleksandriya, with the player joining Dnipro-1 on 1 July once his previous contract expires.

[1] On 24 June 2021, Neven Đurasek was signed on a one-year loan deal from Dinamo Zagreb, with the player joining the club on 1 July once the summer transfer window opens.

[2] On 25 June 2021, Oleksandr Pikhalyonok, who played the previous year on loan from Shakhtar Donetsk, signed a 4-year contract with the team from Dnipro, with the transfer being valued at €700,000.

[3] On 25 June 2021, Andriy Tsurikov signed on a free transfer for Metalist Kharkiv, with the player joining the new club on 1 July after his contract expired.

[4] On 30 June 2021, Nazariy Rusyn was signed on a one-year loan deal from Dynamo Kyiv, with the player joining the club on 1 July once the summer transfer window opens.

[6][7][8][9][10] On 1 July 2021, Bohdan Sarnavskyi was loaned out to Kryvbas Kryvyi Rih on a one-year long deal.

[22] On 27 December 2021, Dnipro-1 announced that Arseniy Batahov will be loaned out to Ukrainian First League club Polissya Zhytomyr until the end of the season.