The 2021 Cholet-Pays de la Loire was the 43rd edition of the Cholet-Pays de la Loire road cycling one day race, which was held on 28 March 2021, starting and finishing in the French town of Cholet, in the Maine-et-Loire department.

The route covers 201.8 kilometres (125.4 mi) and finishes off with seven laps of an 8 kilometres (5.0 mi) long circuit within Cholet.

[3] The race was won in a sprint finish by Elia Viviani (Cofidis) ahead of Jon Aberasturi (Caja Rural–Seguros RGA) and Pierre Barbier (Delko).

[4][5] Initially, Nacer Bouhanni (Arkéa–Samsic) finished third in the race, but he was first relegated and then disqualified following a dangerous sprint, during which he pushed Jake Stewart (Groupama–FDJ) into the barriers, breaking Stewart's hand.

Four teams (Arkéa–Samsic, Bingoal Pauwels Sauces WB, Intermarché–Wanty–Gobert Matériaux, and Sport Vlaanderen–Baloise) entered with seven riders, while all other teams entered with eight riders each.