[1] It served as the season opener of the 2021 IndyCar Series and was the eleventh running of the Honda Indy Grand Prix of Alabama.

Team Penske's Will Power finished second, with defending IndyCar champion Scott Dixon rounding out the podium places in third.

[1] A single red flag was called in the session, when Felix Rosenqvist made contact with the barrier while entering pit road.

[1] The session saw multiple red flags for incidents involving a stall for Dalton Kellett, contact with the Turn 14 wall for Herta, and Newgarden losing power after correcting a slide.

Andretti Autosport driver Alexander Rossi finished first in the second practice session with a time of 01:06.0797, ahead of Chip Ganassi Racing duo Marcus Ericsson and Scott Dixon.