Ashleigh Barty defeated Jil Teichmann in the final, 6–3, 6–1 to win the women's singles tennis title at the 2021 Cincinnati Masters.

Barty did not drop a set en route to her first Cincinnati Masters title.

[2] Victoria Azarenka was the defending champion, but lost in the third round to Barty.

[3] Angelique Kerber contested her 1000th match in her professional career in the quarterfinals against Petra Kvitová; she won the match after Kvitová retired.

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