The runners-up (Connah's Quay Nomads), third-placed team (Penybont), and Europa Conference League play-off winners (Haverfordwest County) qualified for the 2023–24 UEFA Europa Conference League first qualifying round.
[1] The bottom two teams (Airbus UK Broughton and Flint Town United) were relegated to the 2023–24 Cymru North.
Due to 2021–22 Cymru South champions Llantwit Major's failure to achieve a tier one licence, runners-up Pontypridd Town were promoted in their place.
Teams placed 3rd-7th enter one-off play-off matches for the third spot in the Europa Conference League first qualifying round.
Bala Town, Cardiff Metropolitan University, Haverfordwest County, Newtown, and Penybont qualified for the play-offs.