On June 16, Tranmere announced their pre-season schedule.

[39] Win Draw Loss Fixtures Last updated: 18 April 2023 Source: [ Soccerway] Last updated: 29 April 2023.

[41] Rovers were drawn away to Carlisle United in the first round.

[45] Win Draw Loss Fixtures Win Draw Loss Fixtures Tranmere were drawn away to Accrington Stanley in the first round and at home to Newcastle United in the second round.

[46][47] On 20 June, the initial Group stage draw was made, grouping Tranmere Rovers with Bolton Wanderers and Crewe Alexandra.