For transfers in, dates listed are when Detroit City FC officially signed the players to the roster.

Transactions where only the rights to the players are acquired are not listed.

For transfers out, dates listed are when Detroit City FC officially removed the players from its roster, not when they signed with another club.

If a player later signed with another club, his new club will be noted, but the date listed here remains the one when he was officially removed from the Detroit City FC roster.

Source: Match reports As a USL Championship club, Detroit City FC entered the competition in the second round, played April 5–7.