[1][2] Barry Town United were promoted back to the top tier on the first time of asking following their relegation in the 2021–22 season, while Colwyn Bay were promoted to the top tier of Welsh football for the first time since switching from the English football league system in 2018–19.
[3] Flint Town United's relegation was only confirmed on the final day of the previous season, where a dramatic victory for Aberystwyth Town, combined with Flint's defeat to Pontypridd United, saw the Silkmen drop into the relegation zone.
[4] Haverfordwest County came close to being relegated following their failure to achieve a Tier One license at the first time of asking, but their appeal was successful, ensuring their stay in the league.
Teams placed 3rd–7th will enter one-off play-off matches for the third spot in the Conference League first qualifying round.
Since the 2023–24 Welsh Cup winners finished in the top three, the 3rd-placed team qualified automatically for the Conference League and the spot was vacated.