The tournament commenced in December 2023, and concluded with the final on 24 May 2024 at Tottenham Hotspur Stadium, London, England.

[2][3] This is the second year that teams from South Africa can qualify, following the second United Rugby Championship season.

Two teams have been invited from outside the three main European professional leagues; The Black Lion from Tbilisi, Georgia are current holders of the Rugby Europe Super Cup, the third-tier competition in European rugby administered by Rugby Europe, and Cheetahs the current holders of the Currie Cup, South Africa's second tier and most historic national competition.

Location of South African teams Sixteen teams qualified for the 2023–24 EPCR Challenge Cup from Premiership Rugby, the Top 14 and the United Rugby Championship as a direct result of their domestic league performance having not qualified for the Heineken Champions Cup.

The Round of 16 followed a pre-determined format, while the quarter-finals and semi-finals guaranteed home advantage to the higher-ranked team.