This was the second time the tournament features the top teams from South Africa, following the second United Rugby Championship season.

[1] Twenty-four clubs from the three major European domestic and regional leagues qualified to compete in the 2023–24 edition of the Champions Cup.

Note: Placing shown in brackets, denotes standing at the end of the regular season for their respective leagues, with their end of season positioning shown through CH for Champions, RU for Runner-up, SF for losing Semi-finalist, and QF for losing Quarter-finalist.

The knockout stage will begin with the round of 16, starting on 5 April 2024, and concludes with the final on 25 May 2024.

The semi-finals are to be played at a neutral venue, with the higher ranked team having home country advantage.