The Spartans were the reigning champions but were unable to defend their title following their promotion to Scottish League Two.
East Kilbride won a record-equalling third Lowland League title, following a 4–2 victory by Heart of Midlothian B – who cannot win the Lowland League as a guest club[1] – over Bo'ness United on 12 April 2024, which left Bo'ness United nine points behind East Kilbride with two games remaining.
With their scheduled matchup with 2023–24 Highland Football League champion Buckie Thistle being cancelled due to the latter not holding the relevant licence, East Kilbride faced League Two bottom-placed team Stranraer in the final.
They lost 3–5 on aggregate after conceding two goals in the last five minutes of extra time in the second leg at Stair Park.
A three match round robin play-off was scheduled to take place between the winners of the 2023–24 East of Scotland Football League, the 2023–24 South of Scotland Football League, and the 2023–24 West of Scotland Football League, subject to all three clubs meeting the required licensing criteria for promotion.