On 8 June Tranmere Rovers announced their pre-season schedule, with friendlies against Vauxhall Motors, Southport, Fleetwood Town, Rochdale and Everton XI.

[41] Win Draw Loss Fixtures Last updated: 16 March 2024 Source: Soccerway

Source: Soccerway On 22 June, the EFL League Two fixtures were released.

[44] Win Draw Loss Fixtures Rovers were drawn away to Stevenage in the first round.

[49] Win Draw Loss Fixtures In the group stage, Tranmere Rovers were drawn into Northern Group D with Wigan Athletic, Fleetwood Town and Leicester City Under-21s.