A total of 456 teams were accepted into the 2023–24 Women's FA Cup, an increase of 18 from the previous year.

[4] The competition started at the first round qualifying stage with games originally scheduled to be played on 10 September 2023, and made up of teams from outside the top five tiers of the women's football pyramid.

This round included the introduction of teams from the fifth-tier regional first division football leagues.

This round included the introduction of 48 teams from the fourth-tier FA Women's National League Division One.

The first round included the introduction of the 24 teams from the third-tier FA Women's National League Premier Division.