The 2023 Lexington Challenger was a professional tennis tournament played on outdoor hard courts.

It was the 28th edition of the tournament which was part of the 2023 ATP Challenger Tour, and the 26th edition of the tournament which was part of the 2023 ITF Women's World Tennis Tour.

It took place in Lexington, Kentucky, United States between 31 July and 6 August 2023.

The following players received a wildcard into the singles main draw: The following player received entry into the singles main draw using a protected ranking: The following players received entry into the singles main draw as alternates: The following players received entry from the qualifying draw: The following players received a wildcard into the singles main draw: The following players received entry from the qualifying draw: