Each of the five groups of the Segunda División RFEF were represented by the four teams that finished the regular season between the second and fifth positions.
[1] In the draw for the first stage, the participating teams were assigned to pots corresponding to their final regular season position.
[3] The final two relegation spots (of 27 total) were also determined via play-offs.
The four 13th-place finishers with the lowest point totals were drawn into two single-leg matches, with the winners securing survival in the Segunda División RFEF and the losers being relegated to the Tercera División RFEF.
As of this season, the RFEF recovered the two-legged knockout system, due to the complaints filed against the single knockout system at a neutral venue that had been implemented after COVID-19 and the subsequent reform of the football leagues organized by the RFEF.