The top 4 clubs at the end of the regular season move on to the knockout stage where the clubs then play in a knockout tournament, consisting of semi-finals and eventually, the final.

[4] A number of changes to teams competing in this competition happened for this edition.

First, two clubs withdrew from the competition entirely: Argentine-based Jaguares XV and Colombian-based Cafeteros Pro.

They return to the competition they left after the 2020 edition, competing as Ceibos at that time.

[6] Second is another Argentine-based club, Pampas XV, who are competing in the competition for the first time this year.