[3] The incident happened at 19:38 CEST[4] as the bus was transporting tourists from Piazzale Roma in the historic centre of Venice to a campsite in the Marghera district.

[5][6] The bus scraped the guardrail and hit it 27 times[7] along the downhill section of a flyover of a road leading from Mestre to Marghera and the A57 motorway for 50 metres (160 ft) before breaking through the barrier[8] at 6 kilometres per hour (3.7 mph).

[7] The bus fell about 15 metres (49 ft), and landed upside-down with its front completely crushed[8] near the tracks of the Venezia Mestre railway station, where it caught fire.

[9][10] The vehicle, a Yutong E12 model[11] which was operated by La Linea spa (part of Ferrovie Nord Milano group) and rented by a local company to transport the passengers, weighed 13 tonnes and was less than a year old.

[12] Venice's transport councillor later confirmed that the flyover of the accident location had been in need of renovations since 2016, but repairs that were projected to cost €7 million did not begin until September 2023 due to contractor unavailability.

[12] The public prosecutor of Venice opened an investigation into the accident on 4 October,[21] with its coverage to include the state of the flyover's guardrail, an autopsy of the driver, and a search of his phone records.

[25] In March 2024, Allianz has announced its intention to pay the first compensation (300,000 euros per victim), with the Municipality of Venice then reimbursing as responsible for the bad maintenance of the road.

Renato Boraso, Councillor for Mobility and Transport of the Municipality of Venice, criticised the decision of the insurance company, as the accident investigation had not yet established any liability.