Coco Gauff defeated Karolína Muchová in the final, 6–3, 6–4 to win the women's singles tennis title at the 2023 Cincinnati Open.

By reaching the final, Muchová debuted in the top ten of the WTA rankings.

[1] Caroline Garcia was the defending champion,[2] but lost in the second round to Sloane Stephens.

The event is not mandatory on the women's side and points from the 2022 tournament are included in the table below only if they counted towards the player's ranking as of 14 August 2023.

‡ Points from the player's second-best non-mandatory WTA 1000 event, which are required to be counted in her ranking.