Seventeen national relay teams participated in the two heats that took place on 26 August 2023.

The final was won by the Dutch team consisting of Eveline Saalberg, Lieke Klaver, Cathelijn Peeters, and Femke Bol in 3:20.72 minutes, which was a world-leading time and a national record.

In second place finished the Jamaican team with Candice McLeod, Janieve Russell, Nickisha Pryce, and Stacey-Ann Williams in 3:20.88 minutes and third was Great Britain and Northern Ireland with Laviai Nielsen, Amber Anning, Ama Pipi, and Nicole Yeargin in 3:21.04 minutes.

Before the competition records were as follows:[1] After the postponement for COVID-19 pandemic reasons of the 2023 World Relays to 2024 Nassau, the modified system to qualify automatically is to have finished in the first eight (finalists) at 2022 World Championships, in Eugene, completed by eight more 2022-2023 top lists' teams.

[2] Additionally, host country Hungary received a bye for the event.