Beerschot and Dender EH were promoted respectively as champions and runners-up of the 2023–24 Challenger Pro League.
They replaced Eupen and RWD Molenbeek who were relegated at the end of the previous season.
The teams finishing in third and fourth at the conclusion of the relegation play-offs will be relegated to the 2025–26 Challenger Pro League, and the team finishing in second place will have to play the winner of the promotion play-offs, with the winner of that match playing in the 2025–26 Belgian Pro League.
The promotion/relegation play-offs will be contested by the teams finishing in positions 3 to 6 in the 2024–25 Challenger Pro League, who will meet in a knockout tournament with both quarter-finals and semi-finals over two legs, and the highest season finisher always having home advantage in the return leg.
The winner of the semi-finals will play the 14th place finisher of the 2024–25 Belgian Pro League over two legs.