OB and Hvidovre IF finished the 2023–24 season of the Superliga in 11th and 12th place, respectively, and were relegated to the 1st Division.

Esbjerg fB and FC Roskilde won promotion from the 2023–24 Danish 2nd Division.

They replaced Næstved BK and FC Helsingør who were relegated to the 2024–25 Danish 2nd Division.

Note: Flags indicate national team as has been defined under FIFA eligibility rules.

The bottom 6 teams will compete to avoid the 2 relegations to the 2025–26 Danish 2nd Division.