The first team to be relegated in the 2023–24 season was ŁKS Łódź, after a home loss to Śląsk Wrocław on 4 May 2024, ending their one-year stay in Ekstraklasa.

The second relegated team was Ruch Chorzów on 12 May 2024, when Puszcza Niepołomice defeated Warta Poznań; much like ŁKS, they returned to the second tier after just one year in the top-flight.

The last team to be demoted was Warta Poznań, whose fate was sealed after losing to Jagiellonia Białystok on the last matchday, ending their four-year stay.

The first team that won promotion from 2023–24 I liga was Lechia Gdańsk after a win over Wisła Kraków on 11 May 2024, returning to the top flight after a year of absence.

The second promoted team was GKS Katowice, taking the 2nd spot in the table away from Arka Gdynia by defeating them in the last round of the regular season; they returned to the Ekstraklasa after a nineteen-year absence.