The 2024–25 LEN Women's Challenger Cup is the 2nd edition of the water polo competition organised by European Aquatics.

Only countries below the top 6 in the LEN women's club rankings can enter teams into this competition.

The seeding was decided by as follows: Sirens ASC (H) Izmir BSB Spor ZVK Crvena Zvezda ŽAVK Mladost Sport Lisboa e Benfica Jadran Split VK Vojvodina (H) AJF Strakonice (H) City Of Manchester WPC Otter Swimming Club SSV Esslingen SV Blau-Weiss Bochum Sliema ASC Galatasaray Zena Dalton Koleji SK Hapoel Emek Hayarden CSM Unirea Alba Iulia (H) Held in Novi Sad, Serbia

The seeded and unseeded clubs consist of the teams that finished first and second in the previous round respectively.

Izmir BSB Spor Jadran Split ŽAVK Mladost Sport Lisboa e Benfica VK Vojvodina (H) SV Blau-Weiss Bochum Otter Swimming Club Galatasaray Zena (H) Held in Istanbul, Turkey

On 18 January 2025, European Aquatics announced that Galatasaray Zena will be the hosting club for the final four.