East Kilbride are the reigning champions, and they remained in the league having been unable to gain promotion via the League Two play-off for the third time.
East of Scotland League champions Broxburn Athletic were promoted from tier 6, replacing Edinburgh University who had spent 10 seasons in the league.
Relegated to East of Scotland League Promoted from East of Scotland League All grounds are equipped with floodlights.
A three match round robin play-off will take place between the winners of the 2024–25 East of Scotland Football League, the 2024–25 South of Scotland Football League, and the 2024–25 West of Scotland Football League, subject to all three clubs meeting the required licensing criteria for promotion.
However, if no club meets the criteria there will be no promotion to the Lowland League.