[2] The reigning champions entering the season are Northampton Saints, who claimed their second league title after winning the 2024 final.

No team was promoted from the 2023–24 RFU Championship, as the champions, Ealing Trailfinders, were not able to meet the eligibility criteria required to enter the league.

[38] Each team will play one of the play-off legs at their home stadium, with the aggregate score across the two matches determining the winner, who will then compete in the following year's Premiership season.

[67] New to this season is a one-hour highlights programme, titled Gallagher Premiership Unleashed, which is aired on ITV4 each Wednesday at 7pm, recapping all of the fixtures held over the previous weekend.

An abridged, half-hour version of the show is also shown on ITV1 every Wednesday at 11.45pm, while both programmes will remain available to view on demand via ITVX.