[1] The reigning champions entering the season are Gloucester-Hartpury, who claimed their second league title after winning the 2024 final.

The 2024–25 edition of Premiership Women's Rugby is being contested by the same nine clubs which started the 2023–24 season.

[2] No team was brought in to replace Worcester Warriors, who withdrew from the league shortly before the beginning of the previous season.

The schedule is shortened from the previous season, to give national team players more time to rest and prepare for the 2025 Rugby World Cup.

Note: Flags to the left of player names indicate national team as has been defined under World Rugby eligibility rules, or primary nationality for players who have not yet earned international senior caps.