Ealing Trailfinders are the reigning champions, having been refused promotion to the Premiership due to failing to meet minimum standards.

The results of the matches contribute points to the league as follows: Subject to meeting the minimum standards criteria the team finishing top of the league will qualify for a promotion playoff against the bottom side in Premiership Rugby while the team that finishes bottom will be relegated to National League 1.

Eleven of the twelve teams played in last season's competition.

Chinnor were promoted as the champions of the 2023–24 National League 1 with no side being relegated the opposite way due to Jersey Reds going into liquidation in September 2023 and there only being eleven sides.

[1][2] [6] [7] [8] [9] [10] [11] [15] [17] [18][19] [20][21] [23] [24] [6][7][8][9][10][11][15][17][18][20][23][24] Note: Flags to the left of player names indicate national team as has been defined under World Rugby eligibility rules, or primary nationality for players who have not yet earned international senior caps.