[2] The six teams without a SFA club licence were required to be included in the first preliminary round draw – namely Beith Juniors (West of Scotland League champions), Dundee North End (Midlands League champions), Culter (North Region Juniors champions), Cupar Hearts (Amateur Cup winners), Invergordon (North Caledonian League champions), and Bo'ness Athletic (Cup Winners' Shield winners).
Bo'ness Athletic and Dundee North End are taking part in the competition for the first time.
Carluke Rovers (12th in the West of Scotland Fourth Division) were drawn away from home against Buckie Thistle (5th in the Highland League), 89 places above them in the pyramid system when the clubs met, which represented the biggest league position gap between two teams in the competition's history.
The draw for the quarter-finals was made on 10 February 2025 following the St Mirren v Heart of Midlothian match live on BBC Scotland.
[131] The Scottish Cup is broadcast by Premier Sports and BBC Scotland.