The group stage consists of 40 teams: all remaining teams that competed across the SPFL in 2023–24, the 2023–24 Highland Football League champions (Buckie Thistle) and runners-up (Brechin City), and the 2023–24 Lowland Football League champions (East Kilbride).

In addition, for each group stage match that finishes in a draw, a penalty shoot-out took place, with the winner being awarded a bonus point.

The draw for the group stage took place on 29 May 2024 and was broadcast live on the Premier Sports and SPFL YouTube channels.

The draw for the quarter-finals took place on 18 August 2024 following the Celtic v Hibernian match live on Premier Sports 1.

The draw for the semi-finals took place on 22 September 2024 following the Celtic v Falkirk match live on Premier Sports 1.