The league is composed of 60 teams, geographically divided into three different groups.
The group composition was decided and formalized by the Serie C league committee between July and August.
The following teams have changed divisions since the 2023–24 season: Relegated from Serie B Promoted from Serie D Promoted to Serie B Relegated to Serie D On 10 June 2024, the Italian Football Federation rejected Ancona's application due to outstanding debts.
[17][18] On 14 June 2024, the repechage criteria were announced, with new Under-23 teams being given priority to fill the vacancy created by Ancona's exclusion.
Milan Futuro, AC Milan's reserve team, were finally admitted on 27 June.