İstanbulspor was the first team relegated to 2024–25 TFF First League after a 2–1 loss to Fatih Karagümrük at home on 20 April 2024, ending its two-year stay in Süper Lig.
Fatih Karagümrük were themselves relegated after a 3–1 loss at Gaziantep on 18 May 2024, ending their four-year stay in the top flight.
This meant no Ankara team would participate in Süper Lig for at least two years.
The second team promoted is Göztepe, who won 2–0 against Gençlerbirliği at home on 28 April 2024 to return to the top flight after two years.
This meant Muğla would be represented for the first time in the history of the Süper Lig.