[3] Leigh Leopards were the defending champions, having won the 2023 Final, defeating Hull Kingston Rovers 17–16 in extra time.
Toulouse Olympique of the Championship are not full members of the RFL and although invited to take part have declined as in previous years.
Hosted by broadcaster Tanya Arnold, the draw was made by brothers Mike and Steve O'Neill, both of whom played for Widnes in the club's 1983–84 Challenge Cup final victory.
The draw for the semi-finals was made on 14 April, during half time in the match between St Helens and Warrington.
The ties were played on 18 and 19 May as part of a double header, alongside the women's semi finals.